Effect of amnion derived stem cells on inflammatory response in endotoxemic rats.

Abstract

Systemic inflammatory response syndrome (SIRS) and sepsis are one among the most common causes of death in intensive care units. Tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interleukin-10 (IL-10) increase during SIRS and sepsis. Recent studies have demonstrated that amnion derived stem cells have anti-inflammatory effects, low immunogenicity and pluripotency. In addition, there is little ethical objection to the use of amnion derived stem cells. Here, the author presents the first study demonstrating the therapeutic potential for the use of amnion derived stem cells in a rat model of SIRS and sepsis. Amnion derived stem cells were isolated from amnion obtained from an uncomplicated Cesarean section and cultured. SIRS and sepsis were induced in rats by lipopolysacchride (15 mg/kg, LPS), and amnion derived stem cells were then transplanted intravenously. The heart rate, mean arterial pressure and the concentration of TNF-alpha, IL-6 and IL-10 were assessed at baseline and 2 hours and 4 hours after procedure. The changes of heart rate and mean arterial pressure were reduced in the amnion derived stem cells transplanted group. In addition, increases in the plasma concentration of TNF-alpha, IL-6 and IL-10 were attenuated in the amnion derived stem cells transplanted group. This study demonstrated that amnion derived stem cells attenuate inflammatory response during SIRS and sepsis. Transplantation of amnion derived stem cells can be a novel cell based therapeutic strategy for SIRS and sepsis.