Abstract
An experiment was conducted to assess the effect of supplementing α-tocopherol at two different concentrations to the in vitro maturation (IVM) medium (TCM 199 culture medium) on in vitro maturation and cleavage of buffalo oocytes. Ovaries were collected from local slaughter house. Oocytes were collected by aspiration method and were matured in IVM medium (T0, control), IVM medium supplemented with α-tocopherol @ 10 μg/ml (T1) and IVM medium supplemented with α-tocopherol @ 20 μg/ml (T2). The mean cytoplasmic maturation rate was 82.39 ± 0.81, 88.81 ± 1.08, 81.67 ± 1.82 % and nuclear maturation rate was 62.50 ± 8.33, 87.50 ± 5.59, 66.66 ± 7.68 % in T0, T1 and T2 groups, respectively. The fertilized oocytes reaching 2-cell and 4-cell stages of cleavage for T0, T1 and T2 groups were 14.92 ± 1.52, 32.39 ± 1.01 and 16.39 ± 1.25 %, respectively. Significantly (p less than 0.05) higher level of cytoplasmic maturation rate, nuclear maturation rate and cleavage rate was observed in IVM medium supplemented with α-tocopherol @10 μg/ml than other two groups.
Highlights
Inherent reproductive problems limit the productivity of buffalo
Cleavage rate was observed after 48 hr of culture, and it was calculated as: Number of cleaved oocytes (2-4 cell stage embryos) at 48 hr Cleavage rate (%) = X 100 Number of oocytes utilised for maturation in vitro fertilization
The mean cytoplasmic and nuclear maturation rate of buffalo oocytes cultured in in vitro maturation (IVM) medium supplemented with two different concentrations of α-tocopherol are presented in Table 1 and 2, respectively
Summary
Inherent reproductive problems limit the productivity of buffalo. Assisted reproductive technologies such as artificial insemination (AI), superovulation, in vitro fertilization (IVF), and embryo transfer (ET) have been introduced to overcome these problems, to increase the number of offspring from selected females, and to reduce the generation intervals in buffalo. The present experiment was designed with an aim to assess the effect of supplementing α-tocopherol at two different concentrations to the in vitro maturation medium Effect of AlphaTocopherol Supplementation in TCM199 Medium on In Vitro Maturation and Cleavage of Buffalo Oocytes.
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