Abstract
Objective To investigate the effect of all-trans retinoic acid (at-RA) on fetal alveolar epithe-lial type Ⅱ cells (fAECⅡs) proliferation and the expression of pulmonary surfactant C (SPC) as well as aquaporin 5 (AQP5). Methods fAECⅡs were isolated and purified from fetal lung of pregnant SD rats (19 days). After being cultured for 1 day, and the fAECⅡs were interfered by at-RA for 1, 2 and 3 days.Cell proliferation, viability as well as growth state, expressions of SPC mRNA as well as AQP5 mRNA and expressions of protein SPC as well as AQP5 were respectively detected by using 4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide (MTT), inverted microscope, real-time fluorescence quantitative PCR (RT-PCR ) and Western blot. Results (1)When fAECⅡs were treated with at-RA for 1 day, and the cell proliferation and viability did not change (P>0.05), while the proliferation and viability were significantly improved in 2 days (P<0.05), and the difference was the most obvious (P<0.05) at 3 days.(2)Compared with the control group, the cell growth state was better, and the cell adherence was tighter and the refraction was higher in at-RA group.(3)Compared with the control group, at-RA up-regulated the expressions of AQP5 mRNA and AQP5 protein(t=-19.58, -10.44, -16.01, -46.25, -12.79, -27.96, all P<0.05), and the percentages of control group were 281.07%, 766.67%, 1 163.33% and 792.65%, 1 310.52%, 1 561.56% in 1, 2 and 3 days respectively.(4)Compared with control group, the expressions of SP-C mRNA and SPC protein were up-regulated when cells were exposed to at-RA for 1 and 3 d, but while they were down-regulated at 2 days(protein: the percentages of control group were 615.480%, 369.450% and 11.269%, respectively; mRNA: 728.33%, 400.83%, 66.57%, respectively)(t=-26.34, -25.26, 13.80, -25.25, -31.71, 9.12, all P<0.05). Conclusions at-RA can promote the proliferation and differentiation of fAECⅡs, enhance the fAECⅡs viability, and improve the expression of SPC and AQP5. Key words: Retinoic acid; alveolar epithelial type Ⅱ cell; Pulmonary surfactant C; Aquaporin 5
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