Abstract
The present study was carried out to investigating the effect of feeding rats on a balanced diet containing 10% of different oils (exposed to a certain number of fryings) on histological alterations occur in liver, kidney and heart organs. The different suggested treatments that carried out in this investigation could be summarized with their abbreviations as follows: Treatment (T), Control Without any additives, Palm olein (PO), T1 PO+ 0.2% rosemary extracted (RE), T2 PO+ 0.2% Sage extracted (SE), T3 PO+ 0.2% Basil extracted (BE). T4 PO+0.2% Butylated hydraxy touloene (BHT). The palm olein was heated to 60°C before addition of oil extracts (0.2%) rosemary; sage or basil then stirred to ensure that it was completely dissolved. BHT- containing palm olein (0.02%) and control samples (without any antioxidant) were used as positive and negative control. All frying oil samples were heated at frying temperature in about 2 minutes to elevate temperature from 25 to 180°C, followed by addition of potato chips at a rate of 400 g in 5 liters frying oil for 21/2 minutes to complete frying process in the 1st cycle of frying. The 2nd (heating and cooling) cycle of frying process was carried out after 1/2 min. When the frying oil temprature raised again from about 170 to 180°C and potato chips was added at a rate of 400 g. This process was repeated 10 times at the 1st day of the experiment. The experimental ended after 50 frying processes at the 5th day. Samples were withdrawn at 0 time (60°C) then after 10, 30 and 50 frying processes at the 5th day. Samples size was 250ml for biological assay. Results showed that livers of rats fed on diet containing palm oil only used in zero time showed congestion of central vein. While,livers of rats fed on diet containing palm oil only used in 10 times showed hepatocellular necrosis and periductal mononuclear cellular infiltration. Revealed that the livers of rats fed on diet containing palm oil only used in 30 timeshad pronounced periductal mononuclearcellular infiltration. More pronounced alterations were observed in the livers of rats fed on diet containing palm oil only used in 50 timesfibrosis and periductal monocellular infiltration were noticed. The kidneys of rats fed on used palm oil only had also shown significant changes. The livers of rats fed on diet containing palm oil and rosemery only used in 30 times revealed slight vacuolation of hepatocytes. While, the livers of rats fed on diet containing palm oil and rosemery only used in 50 times showed less pronounced periductal fibrosis. Both Sag and basil natural antioxidants administration with palm oil during different times of frying (zero,10,30;50 times) have improved the morphological appearance of hepatocytes but to lesser degree than rosemary. The artificial antioxidants (BHT) did improve also the morphological changes. Rosemary added to palm oil used in zero and 10 times did improve the morphological appearance of both glomeruli, tubules, and interstitial tissue. Both Sag and basil natural antioxidants administration with palm oil during different times of frying (zero,10,30;50 times) have improved the morphological appearance of the kidneys
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