Abstract
IgG and IgM anti-A and/or -B agglutinin titers were determined on 17 serum samples (5 group 0, 7 group A, 5 group B) to range from 8 to 1024. The presence of hemolysins was also evaluated. Single adsorptions with solid-state synthetic A and B substances greatly reduced or eliminated anti-A and -B titers but did not adsorb known platelet antibodies. Unadsorbed and adsorbed serum samples were crossmatched with ABO-compatible and -incompatible platelets by a radioimmunoassay employing 125I-labeled monoclonal antibodies specific for human gamma, mu, and C3d antigens. IgG and IgM crossmatch incompatibility was directly related to ABO alloantibody titers greater than or equal to 64. The use of adsorbed serum in the crossmatch eliminated or greatly reduced incompatible results that were due to ABO alloagglutinins alone, thus allowing the reliable detection of platelet and/or HLA antibodies.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
