Abstract
Mouse peritoneal macrophages (MPM) cultivated with a mouse beta-interferon preparation (MuIFN-beta) or "mock" IFN were tested for phagocytic ability and intracellular levels of adenosine 3',5'-cyclic monophosphate (cAMP). Suspensions of nonopsonized Escherichia coli (E. coli) were used for phagocytosis experiments. Treatment of MPM with 10(1)-10(3) U per ml of MuIFN-beta stimulated the phagocytic activity and raised the levels of cAMP in MPM. The effect of MuIFN-beta on cAMP levels were dose and time dependent. Maximal cAMP levels were seen when MPM were incubated with 10(3)-10(4) U per ml of MuIFN-beta for five hours. Simultaneous addition of MuIFN-beta and the adenylcyclase inhibitor N-ethylmaleimide to the MPM cultures prevented the rise in cAMP levels but not the increased phagocytic capacity. MuIFN-beta induced enhancement of phagocytosis and elevation of cAMP levels in MPM seem to be two parallel but not interlinked processes.
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