Effect of a Misoprostol/Phenytoin Gel on Experimentally Induced Wounds in Brook Trout-A Preliminary Study.

Abstract

Topical treatment for wound management in fish is challenging due to their aquatic life. The objective of the present study was to assess the effect of a topical gel containing misoprostol and phenytoin on the healing of skin wounds in Brook Trout Salvelinus fontinalis. Four 16-×8-mm, full-thickness wounds were created in each trout. Fish were randomly assigned either to the misoprostol/phenytoin group (14 fish) or to the untreated control group (5 fish). In fish from the misoprostol/phenytoin group, two randomly selected wounds were topically treated with a misoprostol/phenytoin gel, while the other two wounds were left without topical treatment. Follow-up and treatment were performed every 5d for 120d. Different macroscopic healing indexes were recorded over time, and histological characteristics of each wound were scored at the end of the study. Treatment with misoprostol/phenytoin was associated with delayed epithelialization and wound maturation in comparison with contralateral untreated wounds. The treatment was also associated with lower histological inflammation scores and increased dermal neovascularization. The untreated wounds of fish in the misoprostol/phenytoin group showed delayed maturation and decreased healing speed and had lower inflammation scores and increased neovascularization compared to untreated control wounds. Topical treatment of wounds in Brook Trout with misoprostol/phenytoin gel at the concentration used had a local and systemic deleterious effect on wound healing. The results of the present study do not support the use of this gel at this dosage for the treatment of wounds in Brook Trout.