Effect exposure of non-specialist tissue to ultraviolet treatments on gene expression and growth under cold stress in some breeds of beans

Abstract

the experiment was conducted in laboratories of institute of genetic engineering and biotechnology of higher studies – university of Baghdad and college of sciences – university of Babylon, to study five breeds of beans to different mutation treatments on shoot apexes and its growth under low heat environments to product new winter lines, the irradiation of shoot apexes was after cut off it from shoots, ultraviolet radiation was used in three wavelengths (220,320 and 400 nm) interaction with two exposure periods (2 and 4 hours per day). the study traits were: Number days to 100% flowering - biological weight– weight of plant seeds, randomized complete block design was used with three replications, the results were indicated that B2 gave less number of days, higher biological and seeds weight while B1,B4 and B5 could not complete growth under W2,W3 treatments with different exposure periods, all breads gave flowering, biological and seeds weight with its variation at P1W1 and P1W2 treatments, the atpA gene was active in all breeds in P1W1 and P1W2 treatments while B2 and B3 were expressed gene in all treatments. From the results, there were new lines of beans added to winter plants as new crop was entered into food diversity program in Iraq.