Efek suplementasi tepung kulit jengkol pada pelepah sawit fermentasi terhadap profil mikroba rumen dan produksi gas metan in vitro

Abstract

<p class="MDPI17abstract"><strong>Objective: </strong>This research was aimed to evaluation the effect of supplementation jengkol peel powder in fermented oil palm fronds on rumen microbes profile and methane production on <em>in vitro</em>.</p><p class="MDPI17abstract"><strong>Methods: </strong>The used design was factorial randomized block design with four treatments supplementation of jengkol peel powder at 0, 2, 4, and 6% and four groups as replications. The observed variables were rumen microbes profile (population of total bacteria, cellulolytic, and protozoa), methane production, partial <em>volatile fatty acid</em> (VFA) proportion and acetate/propionate (A/P) ratio. The data were analyzed using ANOVA and different among treatments means examined by Duncan Multiple Range Test (DMRT).<strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong>The result showed that supplementation of jengkol peel powder at 6% substrate increased (P<0.05) total and cellulolytic bacteria population. Jengkol peel powder supplementation as much as 4 and 6% decreased (P<0.05) protozoa population, methane production, acetate proportion and A/P ratio. Supplementation of jengkol peel powder at 2% increased (P<0.05) propionate production.<strong></strong></p><p class="MDPI17abstract"><strong>Conclusions: </strong>In conclusion, fermented oil palm fronds can be substituted with jengkol peel powder at 2% substrate that did not disturb rumen microbes profile, decreased methane production, acetate proportion, A/P ratio and increased propionate production on <em>in vitro</em>.<strong></strong></p>