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Abstract
This study aimed to evaluate the influence of follicular diameter on the quality of oocytes from ovaries of female sheep and goats. The ovaries of sheep (156 units) and goats (105 units) from slaughterhouse were used. The follicles were measured, aspirated and divided into three classes of follicular diameter: class 1 (CI) – 2 to 3 mm, class 2 (CII) – 4 to 5 mm and class 3 (CIII) – 6 mm. The recovered oocytes were evaluated and classified according to morphological aspect in 5 quality groups: grade 1 (GI), grade 2 (GII), grade 3 (GIII), naked (N) and atresia (A). Of the 468 follicles aspirated from sheep, 327 CI, 84 CII, CIII 57 and 83 GI, 78 GII, GIII 95, 119 N, 93 A were found, and from the 422 follicles aspirated from goats 197 CI, 132 CII, 92 CIII and 64 GI, 70 GII, 91 GIII, 123 N, 74 A were found. The data did not show significant correlation between quality of oocytes and diameter of follicles (P > 0.05). Under the conditions observed in this study it was concluded that the follicular diameter has no influence on the quality of cumulus-oocyte complex (COC) recovered from the ovaries of female sheep and goats.KEYWORDS: Aspirated follicle, cumulus cells, oocyte quality.
Highlights
Nos meados da década de 1980 foram obtidos os primeiros produtos oriundos da fecundação in vitro (FIV) de oócitos ovinos (CHENG et al, 1986) e de oócitos caprinos maturados in vitro (HANADA, 1985)
This study aimed to evaluate the influence of follicular diameter on the quality of oocytes from ovaries of female sheep and goats
Effect of follicle size on bovine oocyte quality and development competence following maturation, fertilization and culture in vitro
Summary
Os ovários utilizados foram obtidos de fêmeas ovinas e caprinas abatidas no abatedouro Suimax, localizado na cidade de Igarassu, Região Metropolitana da Cidade do Recife. Colocaram-se os ovários em recipiente térmico contendo solução salina a 0,9% aquecida a 30oC e acrescida 30 μg/mL de sulfato de gentamicina. Imediatamente foram transportados ao Laboratório de Biotécnica da Reprodução do Departamento de Medicina Veterinária da Universidade Federal Rural de Pernambuco, em tempo não superior a uma hora, após o abate. Os ovários foram lavados em solução salina, mantidos em banho-maria a 37oC e todos os folículos visíveis foram mensurados com paquímetro e divididos em três classes de diâmetro folicular: classe I (CI) – medindo de 2 até 3 mm; classe II (CII) – medindo 4 mm até 5 mm e classe III (CIII) ≥ 6 mm. Após ser realizada essa classificação, procedeu-se à aspiração dos oócitos com o auxílio de seringas de 5 mL e agulhas 19G1/2
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