Editorial: Plant cryobiotechnology: multidisciplinary and modern research to understand the fundamental basis that allows successful cryopreservation and recovery.

Thymus transplantation is indicated for the treatment of athymia and associated T cell deficiency in patients with DiGeorge syndrome. HLA matching of donor and recipient is currently limited by availability...

26. Freezing under pressure: A novel method for cryopreservation

Using microscope for onco-testicular sperm extraction for bilateral testis tumors

BACKGROUND Superior mesenteric artery (SMA) injuries rarely occur during blunt abdominal injuries, with an incidence of < 1%. The clinical manifestations mainly include abdominal hemorrhage and peritoneal irritation, which progress rapidly and are easily misdiagnosed. Quick and accurate diagnosis and timely effective treatment are greatly significant in managing emergent cases. This report describes emergency rescue by a multidisciplinary team of a patient with hemorrhagic shock caused by SMA rupture. CASE SUMMARY A 55-year-old man with hemorrhagic shock presented with SMA rupture. On admission, he showed extremely unstable vital signs and was unconscious with a laceration on his head, heart rate of 143 beats/min, shallow and fast breathing (frequency > 35 beats/min), and blood pressure as low as 20/10 mmHg (1 mmHg = 0.133 kPa). Computed tomography revealed abdominal and pelvic hematocele effusion, suggesting active bleeding. The patient was suspected of partial rupture of the distal SMA branch. The patient underwent emergency mesenteric artery ligation, scalp suture, and liver laceration closure. In view of conditions with acute onset, rapid progression, and high bleeding volume, key points of nursing were conducted, including activating emergency protocol, opening of the green channel, and arranging relevant examinations with various medical staff for quick diagnosis. The seamless collaboration of the multidisciplinary team helped shorten the preoperative preparation time. Emergency laparotomy exploration and mesenteric artery ligation were performed to mitigate hemorrhagic shock while establishing efficient venous accesses and closely monitoring the patient’s condition to ensure hemodynamic stability. Strict measures were taken to avoid intraoperative hypothermia and infection. CONCLUSION After 3.5 h of emergency rescue and medical care, bleeding was successfully controlled, and the patient’s condition was stabilized. Subsequently, the patient was transferred to the intensive care unit for continuous monitoring and treatment. On the sixth day, the patient was weaned off the ventilator, extubated, and relocated to a specialized ward. Through diligent medical intervention and attentive nursing, the patient made a full recovery and was discharged on day 22. The follow-up visit confirmed the patient’s successful recovery.

A man in his mid-40s, diagnosed with motor neuron disease (MND) and Fabry disease (FD), was sent to cardiology after experiencing a cardiac arrest at home secondary to ventricular fibrillation (VF).Fabrydisease is a rare X-linked inherited lysosomal storage disorder caused by deficient alpha-galactosidase A(AGAL-A) activity that leads to an accumulation of globotriaosylceramide (Gb3) in affected tissues, including the heart. Motor neuron disease is an uncommon condition that progressively damages parts of the nervous system. He experienced a positive neurological recovery, enabling the cardiology team to investigate the cause of his arrest.Despite his considerable coexisting health issues and reliance onmedical and non-medical interventions, he seemed to enjoy a good quality of life. By working together, the multidisciplinary team, alongside the patient and their family, reached an agreement on the treatment offered. In light of his ongoing medical conditions, the patient underwent a subcutaneous implantable cardioverter defibrillator (S-ICD) implantation to reduce the risk of infection while ensuring he got the relevant protection in the event of further life-threatening ventricular arrhythmia. He had a successful recovery, maintaining his quality of life and independence.Here, we describe the challengesinvolved in ensuring the best interest decision was taken, which required extensive collaboration from various specialties following a cardiac arrest.

Successful Recovery with Venovenous ECMO for ARDS After LVAD HeartMate 3 Implantation: A Case Report

This case highlights the significant challenges in the diagnosis and management of eumycetoma, particularly in regions like Sudan, where socioeconomic factors and ongoing conflict severely impact patient care. Delayed diagnosis and inadequate access to effective treatment can lead to poor adherence to prescribed therapies, prompting patients to resort to unproven self-treatment methods. Comprehensive, multidisciplinary approaches that include education, improved accessibility to care, and addressing the impact of social determinants on health are essential to enhance the management of mycetoma, reduce disability rates, and improve patient outcomes in underserved communities. Mycetoma is a chronic and debilitating infectious disease characterized by localized swellings and granulomatous lesions. It primarily affects individuals in tropical and subtropical regions and is caused by certain fungi or bacteria. This case report outlines the presentation, diagnosis, and management of a 37-year-old male from central Sudan with black grain eumycetoma, a challenging condition. The patient presented with recurring painless swelling in his right foot, which progressed over 5 years to include sinuses discharging black grain-like materials. Despite initial treatment with itraconazole and folic acid, the patient discontinued medication due to war-induced hardships including financial and accessibility to treatment and healthcare guidance, resulting in resorting to none-effective and potentially harmful herbal remedies. Multidisciplinary management involving dermatologists, infectious disease specialists, and pharmacists supported with community health workers for health education is essential for enforcing adherence to treatment and successful recovery.

“Growth plate fracture of the proximal tibia in a young adult with an unfused growth plate: A case report”

Laryngeal fractures are life-threatening injuries, frequently associated with long-term morbidity. We present a case of a man sustaining a displaced laryngeal fracture and rupture of supraglottic structures following attempted suicide...

Introduction to a <i>Virtual Special Issue</i> on plant ecological strategy axes in leaf and wood traits

Science, interdisciplinarity, and the society

Successful recovery from COVID-19 pneumonia after receiving baricitinib, tocilizumab, and remdesivir. A case report: Review of treatments and clinical role of computed tomography analysis

A female patient in her 30s presented to the emergency department with a 10-day history of fever, weakness and diaphoresis. Subsequent investigations revealed a diagnosis of haemophagocytic syndrome, secondary to...

Successful cryopreservation of plant shoot tips is dependent upon effective desiccation through osmotic or physical processes. Microscopy techniques were used to determine the extent of cellular damage and plasmolysis that occurs in peppermint (Mentha x piperita) shoot tips during the process of cryopreservation, using the cryoprotectant plant vitrification solution 2 (PVS2) (30% glycerol, 15% dimethyl sulfoxide, 15% ethylene glycol, 0.4 M sucrose) prior to liquid-nitrogen exposure. The meristem cells were the smallest and least plasmolyzed cell type of the shoot tips, while the large, older leaf and lower cortex cells were the most damaged. When treated with cryoprotectant solutions, meristem cells exhibited concave plasmolysis, suggesting that this cell type has a highly viscous protoplasm, and protoplasts have many cell wall attachment sites. Shoot tip cells were most severely plasmolyzed after PVS2 treatment, liquid-nitrogen exposure, and warming in 1.2 M sucrose. Successful recovery may be dependent upon surviving the plasmolytic conditions induced by warming and diluting treated shoot tips in 1.2 M sucrose solutions. In peppermint shoot tips, clumps of young meristem or young leaf cells survive the cryopreservation process and regenerate plants containing many shoots. Cryoprotective treatments that favor survival of small, meristematic cells and young leaf cells are most likely to produce high survival rates after liquid-nitrogen exposure.

This paper provides an introduction to a long-term biological monitoring program and the Environmental Management special issue titled Long-term Biological Monitoring of an Impaired Stream: Implications for Environmental Management. The Biological Monitoring and Abatement Program, or BMAP, was implemented to assess biological impairment downstream of U.S. Department of Energy (DOE) facilities in Oak Ridge, Tennessee, beginning in 1985. Several of the unique aspects of the program include its long-term consistent sampling, a focus on evaluating the effectiveness of specific facility abatement and remedial actions, and the use of quantitative sampling protocols using a multidisciplinary approach. This paper describes the need and importance of long-term watershed-based biological monitoring strategies, in particular for addressing long-term stewardship goals at DOE sites, and provides a summary of the BMAP's objectives, spatial and temporal extent, and overall focus. The primary components of the biological monitoring program for East Fork Poplar Creek in Oak Ridge, Tennessee are introduced, as are the additional 9 papers in this Environmental Management special issue.