Edaravone's reno-protective effects against chronic heat-stress exposure.

Abstract

Edaravone (EDV) is a potent antioxidant with anti-inflammatory properties. It is used to treat various diseases, especially neurodegenerative diseases. This study aims to examine EDV's potential renal protective effects on kidney injury induced by heat stress in rats. Male Wistar rats were segregated into four distinct groups (n = 16/group): control (Ctr), heat stress (HS), edaravone (EDV), and HS+EDV groups. Heat stress was applied 6 days a week for 30 min for 8 weeks, and EDV treatment (6 mg/kg. IP) was administered simultaneously in the HS+EDV group. After the experiment, blood and kidney tissue samples were gathered for subsequent analysis. Compared to the control group, the HS group exhibited a significant increase in serum creatinine and urea levels (P < 0.05). Additionally, malondialdehyde level and catalase activity, tumor necrosis factor-α (TNF-α), and interleukin-1 beta (IL-1β) mRNA expression were increased in the kidney tissue during HS. The renal tissues of the heat-stressed animal showed noticeable histological alterations compared to the control group. However, in the HS+EDV and EDV groups, the creatinine and urea concentrations in the blood were markedly reduced compared to the HS group (P < 0.05). In addition, renal oxidative stress biomarkers were normalized (malondialdehyde levels and catalase activity; P < 0.05). The histopathological alterations in the renal tissues of the groups treated with EDV were markedly diminished. In addition, the renal mRNA expression levels of IL-1β and TNF-α were markedly reduced in the HS+EDV group compared to the HS group (P < 0.05). EDV treatment in a heat-stress rat model demonstrated a protective effect on renal tissue, most likely due to its antioxidant and anti-inflammatory properties.