Abstract
YABBY gene family plays an important role in the polarity development of lateral organs. We isolated the BraYAB1-702 gene, a member of the YABBY gene family, from young leaves of Chinese cabbage line 06J45. The full-length gene has a 937 bp CDNA sequence and contains an open reading frame (ORF) of 702 bp. The subcellular localization analysis showed that the expression product of the gene was localized in the nucleus. Ectopic expression of BraYAB1-702 in Arabidopsis thaliana caused leaf curling from the adaxial epidermises to abaxial epidermises; the partial abaxialization of the adaxial epidermises of leaves; leaf trichomes and stomata numbers being significantly increased; the plants being severely stunted; the flowering stage being remarkably delayed and inhibiting the development of shoot apical meristem (SAM) with the down-regulation of the expression of SHOOT MERISTEMLESS (STM), Brevipedicellus (BP) and KNAT2 which were related to the development of shoot apical meristem. These results from the present research help to reveal the molecular mechanism of BraYAB1-702 gene in the establishment of adaxial–abaxial polarity of the lateral organs in Chinese cabbage.
Highlights
In higher plants, lateral organs are initiated from the peripheral zone of shoot apical meristem (SAM)
To study the molecular mechanism of inhibition of the growth of SAM in transgenic Arabidopsis thaliana plants, the primers for SHOOT MERISTEMLESS (STM), BP and KNAT2 genes related to the development of SAM of Arabidopsis thaliana and ACTIN8 was used as the reference gene (Table 1) were designed for quantitative analysis
BraYAB1-702 is a member of the YABBY gene family and it is expressed in the lateral organs of Chinese cabbage
Summary
Lateral organs are initiated from the peripheral zone of shoot apical meristem (SAM). No sequence homology is found outside the zinc finger-like domain and the YABBY domain All of these genes are expressed in the abaxial regions of lateral organs, each has the specific expression pattern [2,3,9]. The YABBY genes in Arabidopsis thaliana are related to abaxial sides’ characteristics, they are expressed in the adaxial sides of the leaves in maize, which is a monocotyledon [13]. OsYAB1 gene in rice is not expressed in a polar manner in lateral organs, it is the homology to YAB2 gene in Arabidopsis thaliana [6] Taken together, these results indicate that the expression and functions of YABBY genes is not entirely conserved in all species, and have diverged between monocotyledons and dicotyledons [14]. Arabidopsis thaliana, in order to deepen the understanding of the functions of this gene and explore new materials for the study of the development of Chinese cabbage leaves
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