Abstract
Melissa officinalis L. is a plant with different medicinal and culinary uses, which is considered one of the essential oils with the highest market value, mainly because of its low yield. The use of technologies that improve the extraction of this essential oil is justified. The study evaluated the use of cell wall degrading enzymes (CWDE) obtained from Pleorotus djamor to extract essential oil of Melissa officinalis L.. For the optimization of the enzymatic pre-treatment, the independent variables time and CWDE/H2O ratio were evaluated through central composite rotational design (CCRD). The essential oil extraction was carried out by hydrodistillation after pre-treatment. The results showed that the pre-treatment was able to increase the essential oil yield by 71 % and reduced energy consumption and CO2 emissions by 42 %, when applied for 60 minutes and CWDE/H2O ratio (25/450 mL.mL−1). Gas chromatography-mass spectrometry (GC/MS) was used to evaluate the essential oil composition. The majority of compounds were citral (Neral +Geranial). In addition, the increase of this compound was observed at about 24 %. The lemon balm plant cell wall was degraded. Considering the production and energy costs and increase yield as well as the quality of lemon balm essential oil, the application of CWDE was efficient.
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