Ecological evolution during the three-year restoration using rhizosphere soil cover method at a Lead-Zinc tailing pond in Karst areas

Abstract

A major challenge for the restoration of the Lead-Zinc tailing pond in Karst areas lies in how to establish vegetation with less soil and restore the ecological functions of the substrate. In this study, a novel method, rhizosphere soil cover method (RSC), was applied to recover the vegetation at a Pb-Zn tailing pond in Karst areas. Two local tolerate plants, Miscanthus sinensis and Pueraria phaseoloides, were planted as pioneer species. Although 68 % of the tailing pond was not covered with soil, the vegetation coverage has reached over 90 % after restoration for three years. Compared with the natural revegetation process (vegetation coverage was <5 % after 20 years of natural succession), the revegetation in the tailing pond was accelerated by RSC and planting pioneer species. Both the plant's diversity and richness have significantly increased in the tailings pond during the restoration (p < 0.05). The important value indicators of M. sinensis and P. phaseoloides were the highest in the plant community, indicating the dominant role of these two plants in revegetation. Moreover, the total organic carbon, total nitrogen, total phosphorus, and total potassium in the tailings increased annually (p < 0.05), which demonstrated that the revegetation has improved the chemical properties in the substrate. In addition, the Shannon diversity index of bacteria in the tailings increased significantly from 4.11 to 5.51. The relative abundance of microbial genes related to carbon fixation and nitrogen fixation in the tailings increased by 17 % and 43 %, respectively. Meanwhile, the physicochemical properties, microbial community structure, and nutrient cycling function in the tailings without topsoil were improved more obviously than those in soils. It is thereby concluded that RSC is an efficient means for ecological restoration of the tailing ponds in Karst areas to improve the ecosystem structure and function of Pb-Zn tailings.