Abstract
A distinct grain size of 600, 425 and 212 μm sand column was developed to test the efficiency of bacterial strain Sporsarcina pausterii NIOT-1. The bacterial strain was effectively able to precipitate calcite crystals between the soil particles through enzymatic hydrolysis of urea which increases ammonium concentration to the maximum of 371.43 mM, 314.54 mM, 302.49 mM, EC of 98.81 ms/cm, 101.84 ms/cm, 101.34 ms/cm and pH of 8.80, 8.90, 8.80 respectively. The synthesized biomaterials have the maximum UCS of 2400 Mpa with 212 μm grain size followed by 425 and 600 μm with 2200 and 2100 Mpa. Scanning Electron Microscopy and XRD results justify the precipitation of CaCO3 bridging the sand particle. In the study, the maximum UCS attained with fine sand particle size and reduced with the increasing grain size. The current study observed an inverse relationship between grain size and strength. The current green synthesis study also attempts for the feasibility approach to reduce coastal erosion.
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