Abstract
Summary1. Two shipments of radio-iodinated human serum albumin (RISA), produced by different commercial laboratories, were analysed by ascending and circular chromatography, electrophoresis, autoradiography and radioactive scanning.2. Both shipments were subdivided into three samples and each kept under slightly different conditions, in order to study the best conditions for storage of the product.3. Results obtained showed progressive albumin denaturation in both shipments, with the formation of radioactive degradation products and radioactive iodide. This process of radiolysis was less intense in the samples maintained permanently in the refrigerator and handled as little as possible.4. The extent of alteration induced was smaller when the protein concentration was around 5 mg per ml and the initial radioactivity less than 0.8 mCper ml.5. The results obtained show the need for a frequent control of the albumin, not only in relation to the iodide liberated, but also with respect to degradation of the albumin by radiolysis.6. Circular chromatography followed by autoradiography is the simplest method of control which can be easily performed in any clinical laboratory.
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