Abstract
Abstract: Adoption of a standard procedure to perform the germination test allows comparison of results between laboratories. Thus, the aim of this study was to evaluate the germination process of rangpur lime (Citrus limonia Osbeck) seeds conducted under different temperatures. The germination test was carried out in five seed lots under three temperatures (25, 30 and 35 °C). The dynamics of the germination process was studied through the following variables: germination (%), time to first germination (days), time to last germination (days), time interval between first and last germination (days), time required to reach 50% of germination (days), mean speed of germination (day-1), synchrony of the germination process (dimensionless), and uncertainty of the germination process (bits). The experimental design was completely randomized, and analysis of variance was performed in a 5 (lots) x 3 (temperatures) factorial arrangement, with four replicates of each treatment. Means were compared using Tukey’s test (p < 0.05). The germination process in rangpur lime seeds is slow, homogenous, and asynchronous; and the temperature of 30 °C is the most favorable for conducting the germination test, with the first count at 21 days and the last at 30 days.
Highlights
Seed commercialization is directly governed by seed lot viability data, which is obtained by germination and/or tetrazolium tests (Brasil, 2013)
The germination test was conducted in a paper roll substrate (PR) with four replications of 50 seeds, arranged on three sheets of paper for germination moistened with distilled water at the proportion of 2.5 times the dry weight of the paper
These results show that conducting the germination test of rangpur lime seeds is negatively affected at the temperature of 35 °C, since under this temperature condition, seedling abnormalities were identified and the occurrence of dead seeds
Summary
Seed commercialization is directly governed by seed lot viability data, which is obtained by germination and/or tetrazolium tests (Brasil, 2013). From these results, the seed producer can compare the physiological quality of seed lots as a parameter for seed commercialization. The germination test is performed under controlled conditions of moisture, temperature, and lighting, and other necessary conditions. These requirements allow germination to occur in a regular, fast, and complete manner, ensuring that the test can be reproduced and that its results can be used for comparison (Brasil, 2009). For rangpur lime (Citrus limonia Osbeck), no method has yet been described
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