Abstract
It is becoming clear that the structure of the cell is important for the organization and energy metabolism of cells. Previous studies in our lab showed how mitochondria in adult cardiomyocytes are arranged regularly within Intracellular Energetic Units in a longitudinal lattice at the level of A-band between the myofibrils and located within the limits of the sarcomers (Biochim Biophys Acta, 2010, 1797: 678-97). in the present study the mitochondrial position and dynamics were studied in adult rat cardiomyocytes in which Z-lines were labelled by fluorescent α-actinin. Fast scanning of intact adult cardiomyocytes by transfected GFP-α-actinin was carried out using a line scanning LSM7 LIVE and LSM710 confocal microscope. The results show that cytoskeletal proteins position each mitochondrion in a specific area and make fusion and fission physically unfeasible processes. The fast and simultaneous line scanning in two fluorescent channels synchronized with the piezo stage axial displacements allow rapid 3D imaging of the mitochondrial dynamics referenced to the structural elements of sarcomers. The on-going research work aims at capturing dynamic behaviour of both intra- and extra-mitochondrial regulatory proteins in correlative manner.This study was performed in the framework of ANR project SYBECAR, France.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
