Biochemical and biophysical research communications | VOL. 609
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Dynamic surface tension probe for measuring the concentration of extracellular vesicles.

Publication Date Jun 18, 2022

Abstract

The concentration of extracellular vesicles (EVs) is an essential attribute of biofluids and EV preparations. EV concentration in body fluids was correlated with health status. The abundance of EV secreted by cultured cells into growth medium is vital in signaling studies, tissue and disease models, and biomanufacturing of acellular therapeutic secretome. A limited number of physical principles sensitive to EV concertation have been discovered so far. Particle-by-particle counting methods enumerate individual particles scattering light, modulating the Coulter current, or appearing in EM images. The available ensemble techniques in current use rely on the concentration-dependent signal intensity, as in the case of ELISA. In this study, we propose for the first-time the ensemble-based characterization of EV concentration by dynamic surface tension (DST) probe and demonstrate its implementation. We show that DST measurements agree with the widely used NTA measurements of EV concertation. The proposed method is low-cost and requires only basic laboratory equipment for implementation.

Concepts
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Extracellular Vesicles
Dynamic Surface Tension
Concentration Of Extracellular Vesicles
Concentration In Body Fluids
Extracellular Vesicles Preparations
Tension Probe
Concentration Of Vesicles
EM Images
Ensemble Techniques
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