Abstract
Objective To study the expression of CaSR in renal calculi by injecting nanoparticles into the tail vein and to investigate the role of CaSR in the formation of gallstones. Methods A total of 60 Wistar male rats were randomly divided into normal control group and nanobacterial group, with 30 rats in each group. The rats in the normal control group were given disposable intravenous injection with 1.2 mL of 0.9% sodium chloride solution via the tail vein, the rats in the nanobacterium group were given disposable intravenous injection with 1.2 mL of nanobacterial suspension via the tail vein at one time. Three rats in each group were sacrificed every week from the 1th to 10th week after injection. The gross morphology and crystal granule formation of the kidney were observed by using an anatomical microscope. Histopathological examination was performed every week to evaluate the stone formation in 3 male Wistar rats, and the expression of CaSR was detected by immunohistochemistry for 10 weeks. Results ①Pathological examination results: From the 4th to 10th week, the crystal particles were found in the renal tubules of Wistar male rats in the nanobacterial group, and mainly distributed in the proximal and distal convoluted tubules. from the 1th to 10th week, the Wistar rats in the control group had no crystal granules.②The results of immunohistochemistry: Persistent expression of CaSR in nanobacterial group from the 1th to 10 th week. From the 1th to 3th week, there was no significant difference between the nanobacterial group and the control group, but the expression intensity increased gradually and the expression intensity was significantly higher than that in the control group from the 4th to 10th week. From the 1th to 10th week, CaSR in the control group while also sustained expression, but its strength was no obvious change. From the 1th to 10th week, although the expression of CaSR was persistent in the control group, there was no significant change in its intensity. Conclusions The increased activity of CaSR may play an important role in the formation of Nanobacteria renal stone. Key words: Kidney Calculi; Nanostructures; Disease Models, Animal; Receptors, Calcium-Sensing
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