Abstract
The coherent noise always exists in digital holographic microscopy due to the laser source, degrading the image quality. A method of speckle suppression using the dynamic speckle illumination, produced by double-moving diffusers, is presented in digital holographic microscopy. The space–time correlation functions are theoretically analyzed from the statistics distribution in the doubly and singly scattered system, respectively. The configuration of double-moving diffusers is demonstrated to have better performance in speckle suppression compared with the single diffuser and moving-static double diffusers cases. The experiment results verify the feasibility of the approach. The presented approach only requires a single shot interferogram to realize the speckle reduction, accordingly it has the potential application in real-time measurement.
Highlights
Digital holographic microscopy is a powerful technique for quantitatively measuring the three-dimensional (3D) morphology of transparent samples such as biological cells [1,2,3].The laser source, as a coherent illumination, causes the holographic imaging to suffer from the coherent noise
The spatial correlation properties and the temporal correlation properties are analyzed theoretically from the space–time correlation function of double diffusers and single diffuser, respectively. It is demonstrated by the results of simulations and experiments that the doubly scattered system, made up of two moving diffusers, provides better speckle suppression performance compared with the single diffuser or moving-static double diffusers system
Thedouble doublediffusers, diffusers,mounted mountedinintwo twodc dcmotors, motors,rotate rotate digital at certain velocity to generate the large number of decorrelation speckle patterns
Summary
Digital holographic microscopy is a powerful technique for quantitatively measuring the three-dimensional (3D) morphology of transparent samples such as biological cells [1,2,3]. The dynamic speckle field illumination through single moving diffuser [15,16] or double diffusers with a static and a moving state [17] only requires a single shot recording to realize the coherent speckle suppression. The spatial correlation properties and the temporal correlation properties are analyzed theoretically from the space–time correlation function of double diffusers and single diffuser, respectively. It is demonstrated by the results of simulations and experiments that the doubly scattered system, made up of two moving diffusers, provides better speckle suppression performance compared with the single diffuser or moving-static double diffusers system
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