Abstract
Coherent anti-Stokes Raman scattering (CARS) microscopy was used to disclose dynamic information from lipid droplets (LDs) in living cancer cells. Statistical analyses of LD dynamics show different pools of LDs associated with LD synthesis and degradation processes. We found that each LD population satisfies a log-normal distribution, thus allowing quantitative comparison through fitting of population histograms. Two-dimensional analyses of LD displacements and speeds offer dynamic signatures of cells to monitor responses to stimuli and drugs. We revealed hypothermia-induced cellular metabolic changes and a two-step metabolic response during apoptosis. We also explored dynamic signatures of LDs during starvation and various drug exposures.
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