Abstract
An increase in cytosolic Ca2+concentration periphagosomally is critical for phagolysosomal formation and neutrophil elimination of microbes. The Ca2+increase could be achieved through release of Ca2+from mobilized intracellular stores. Alternatively, Ca2+that passively enter the phagosome during phagocytosis could be provided by the phagosome. Intraphagosomal Ca2+changes in single human neutrophils was measured during phagocytosis of serum opsonized Fura-2-conjugated zymosan particles, using a digital image processing system for microspectrofluorometry. A decrease in phagosomal Ca2+down to nanomolar concentrations was seen within minutes following phagosomal closure. Blockage of plasma membrane Ca2+channels by econazole abolished this decrease. The fluorescence properties of Fura-2 zymosan were retained after phagocytosis and stable to pH changes, reactive oxygen species, and proteolytic enzymes. We suggest that Ca2+ions present in the phagosome enter the cell cytosol through Ca2+channels in the phagosomal membrane, achieving a localized Ca2+rise that is important for phagosome processing.
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