Abstract
In biomedical laboratories, an important part of work consists certainly in counting biological particles. The use of ultrasonic waves are particularly of interest because of their harmless towards biological tissues and their ability to control opacous suspension. In this paper, we will show that high frequency ultrasounds (about 100 MHz) are convenient for particles detection. The experimental results obtained with a culture of protozoa are compared to those given by simulation of echograms using matrix transfert formalism where the cell is asumed to be a multilayer structure in which the attenuation is taken into account in the different layers
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