Abstract
Bacteriophages and their hosts are continuously engaged in evolutionary competition. Here we isolated a lytic phage phiYe-F10 specific for Yersinia enterocolitica serotype O:3. We firstly described the phage receptor was regulated by DTDP-rhamnosyl transferase RfbF, encoded within the rfb cluster that was responsible for the biosynthesis of the O antigens. The deletion of DTDP-rhamnosyl transferase RfbF of wild type O:3 strain caused failure in phiYe-F10 adsorption; however, the mutation strain retained agglutination with O:3 antiserum; and complementation of its mutant converted its sensitivity to phiYe-F10. Therefore, DTDP-rhamnosyl transferase RfbF was responsible for the phage infection but did not affect recognition of Y. enterocolitica O:3 antiserum. Further, the deletions in the putative O-antigen biosynthesis protein precursor and outer membrane protein had no effect on sensitivity to phiYe-F10 infection. However, adsorption of phages onto mutant HNF10-ΔO-antigen took longer time than onto the WT, suggesting that deletion of the putative O-antigen biosynthesis protein precursor reduced the infection efficiency.
Highlights
The primary determinant in the infection of a bacterial host by a bacteriophage is the adsorption of the phage to the host receptor
We are first to identify DTDP-rhamnosyl transferase RfbF as the receptor regulator protein, and another protein in a Y. enterocolitica O:3 strain is related to phiYe-F10 adsorption
Bacteriophages are capable of rapid adaptive responses to evolutionary changes in their hosts
Summary
The primary determinant in the infection of a bacterial host by a bacteriophage is the adsorption of the phage to the host receptor. This receptor recognition is the most reported in phage- host interaction studies[1,2]. Y. pseudotuberculosis enterocolitica LPS has three primary components: lipid A, core oligosaccharide and O-side-chain (O-antigen). Published assays showed that we can detect the serotypes of Y. enterocolitica strains using amplification of O-antigen-encoded genes[28]. We are first to identify DTDP-rhamnosyl transferase RfbF as the receptor regulator protein, and another protein (putative O-antigen biosynthesis protein precursor) in a Y. enterocolitica O:3 strain is related to phiYe-F10 adsorption
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