Drug sensitivity Profile of Pseudomonas spp. isolated from a tertiary care hospital against commonly used antibiotics

Abstract

Background: Antimicrobials agents are being abundantly used to control bacterial growth, like Pseudomonas, leading to resistance. Objective: To find out the drug sensitivity patterns of the Pseudomonas spp. against various antibiotics in the tertiary care hospital (Ghurki Trust & Teaching Hospital). Methodology: This cross-sectional study was designed on the retrospective collection of the records of 1000 sample reports, of the patients admitted in Ghurki Trust Teaching Hospital from the period of January 2018 to December 2019, who presented with multiple infections or developed pseudomonas infections during their stay in the hospital. Tests were carried out in the clinical microbiology Laboratory of Lahore Medical & Dental College for bacterial culture. Specimen included were breast tissues, pus, urine and stool culture, blood culture, wound discharge, ear discharge, abscess from the wound, various infected implants, and dead necrotic tissues from bone. Data were analyzed using SPSS version 26. Results: Out of the 1000 samples, a total of 150 (15%) were positive for bacterial growth. Among these 150, 70 were positive for gram-negative. The bacterial profiles of these 150 samples have shown that frequently (42) isolated gram-negative bacteria was Pseudomonas. On further tests, these bacteria were more sensitive to Imipenem and Cilastatin (69%) followed by levofloxacin ( 31%). No sensitivity was found against vancomycin and amoxicillin. Conclusion: In this study, the Pseudomonas were isolated and showed sensitivity to Imipenem and Cilastatin followed by levofloxacin. These studies should be conducted frequently to keep a track of the sensitivity patterns of bacteria.