Abstract
Xylaria sp. BCC 1067 is a wood-decaying fungus which is capable of producing lignocellulolytic enzymes. Based on the results of a single-molecule real-time sequencing technology analysis, we present the first draft genome of Xylaria sp. BCC 1067, comprising 54.1 Mb with 12,112 protein-coding genes.
Highlights
The libraries were sequenced on 16 single-molecule real-time (SMRT) cells using a PacBio RS II sequencer according to the manufacturer’s instructions (Pacific Biosciences, Menlo Park, CA, USA)
A total of 8,912,350,715 bases of genome sequences with 45,112 circular consensus sequence (CCS) reads were assembled using the SMRT Analysis Hierarchical Genome Assembly Process 3 (HGAP3) pipeline, which resulted in 43 contigs, with the length of the longest contig and the N50 value being 6,684,005 bp and 5,573,684 bp, respectively
The analysis revealed that the Xylaria sp
Summary
Genome sequencing was performed using PacBio single-molecule real-time (SMRT) sequencing technology. High-molecular-weight genomic DNA was sheared and selected on a BluePippin system using a cutoff range of 15 to 50 kb. The libraries were sequenced on 16 SMRT cells using a PacBio RS II sequencer according to the manufacturer’s instructions (Pacific Biosciences, Menlo Park, CA, USA). A total of 8,912,350,715 bases of genome sequences with 45,112 circular consensus sequence (CCS) reads were assembled using the SMRT Analysis Hierarchical Genome Assembly Process 3 (HGAP3) pipeline, which resulted in 43 contigs, with the length of the longest contig and the N50 value being 6,684,005 bp and 5,573,684 bp, respectively.
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