Abstract
ABSTRACTThe draft genome sequence of Clostridium tertium WC0709, a gut bacterium able to use mucin in pure culture as the sole carbon and nitrogen source, is presented here. The genome sequence of C. tertium will provide valuable references for comparative genome analysis and for studying the relationship with the host.
Highlights
Clostridium tertium was first described by Henry in 1918 as Bacillus tertius [1] and was reclassified as Clostridium tertium in 1923 [2]
The strain was cultivated in minimal medium (MM) at 37°C for 48 h under strictly anaerobic conditions
Biomass was collected by centrifugation, and the genomic DNA was extracted with a DNeasy blood and tissue kit (Qiagen GmbH, Düsseldorf, Germany)
Summary
Clostridium tertium was first described by Henry in 1918 as Bacillus tertius [1] and was reclassified as Clostridium tertium in 1923 [2]. The strain was cultivated in minimal medium (MM) at 37°C for 48 h under strictly anaerobic conditions. Biomass was collected by centrifugation, and the genomic DNA was extracted with a DNeasy blood and tissue kit (Qiagen GmbH, Düsseldorf, Germany). Before DNA purification, the pretreatment for Gram-positive bacteria was followed by incubation for 2 h at 37°C with 2-fold the volume of enzymatic lysis buffer and for 1 h at 56°C with 2-fold the volume of proteinase K and buffer AL.
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