Abstract

We report the draft genome sequence of Pseudomonas putida JLR11, a facultative anaerobic bacterium that has been studied in detail for its capacity to use the explosive 2,4,6-trinitrotoluene (TNT) as a nitrogen source. The sequence confirms the mechanisms used by this versatile strain to reduce and assimilate nitrogen from TNT.

Highlights

  • We report the draft genome sequence of Pseudomonas putida JLR11, a facultative anaerobic bacterium that has been studied in detail for its capacity to use the explosive 2,4,6-trinitrotoluene (TNT) as a nitrogen source

  • Pseudomonas putida JLR11 was originally isolated from a water treatment plant in Granada (Spain) as a bacterial strain capable of growth with 2,4,6-trinitrotoluene (TNT) as a sole nitrogen source under anaerobic conditions [1], using the nitrite released during the reduction of the TNT as a final electron acceptor for respiratory chains [2]

  • Automated genome annotation was carried out using the NCBI Prokaryotic Genomes Annotation Pipeline (PGAP) [13], as well as the Integrated Microbial Genomes Expert Review (IMG-ER) platform [14]

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Summary

Introduction

We report the draft genome sequence of Pseudomonas putida JLR11, a facultative anaerobic bacterium that has been studied in detail for its capacity to use the explosive 2,4,6-trinitrotoluene (TNT) as a nitrogen source. The sequence confirms the mechanisms used by this versatile strain to reduce and assimilate nitrogen from TNT. Pseudomonas putida JLR11 was originally isolated from a water treatment plant in Granada (Spain) as a bacterial strain capable of growth with 2,4,6-trinitrotoluene (TNT) as a sole nitrogen source under anaerobic conditions [1], using the nitrite released during the reduction of the TNT as a final electron acceptor for respiratory chains [2].

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