Abstract

Here, we present the draft genome sequence of strain UYCP14C, a rhizobium isolated from Calliandra parvifolia nodules. The assembled genome size was around 9.8 million bp, containing 9,031 predicted protein-coding sequences, including several symbiotic and nitrogen fixation genes. UYCP14C appears to be a novel species of the plant growth-promoting Paraburkholderia genus.

Highlights

  • We present the draft genome sequence of strain UYCP14C, a rhizobium isolated from Calliandra parvifolia nodules

  • The most similar sequence (99.49%) corresponded to Paraburkholderia monticola strain JC2948T

  • The UYCP14C genome was assembled with SPAdes v3.9.0 [17], keeping paired reads unmerged before assembly

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Summary

Introduction

We present the draft genome sequence of strain UYCP14C, a rhizobium isolated from Calliandra parvifolia nodules. Root nodule collection and symbiotic bacterial isolation were performed as previously described [12, 13]. UYCP14C identification was performed at EzBioCloud [14] using the PCR-amplified 16S rRNA gene sequence. The most similar sequence (99.49%) corresponded to Paraburkholderia monticola strain JC2948T. Genomic DNA was isolated from stationary-phase cultures in liquid tryptone-yeast (TY) medium [15] using the ZR fungal/bacterial DNA kit (ZymoResearch).

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