Abstract
ABSTRACTHere, we report a draft genome sequence of Mycobacterium peregrinum obtained from a sputum sample of a South African HIV-infected patient with suspected pulmonary tuberculosis. The genome described here comprises 6,931,852 bp, revealing 66.2% G+C content, 6,808 coding sequences, and 81 RNA genes.
Highlights
We report a draft genome sequence of Mycobacterium peregrinum obtained from a sputum sample of a South African HIV-infected patient with suspected pulmonary tuberculosis
Genomic DNA was extracted from three independent liquid cultures using the NucliSENS easyMAG; in brief, a 200-l aliquot of the liquid culture was used as the input volume for DNA extraction using the generic protocol, with a final elution volume of 25 l
All contigs were submitted to GenBank, where gene annotation was implemented using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) [3]
Summary
We report a draft genome sequence of Mycobacterium peregrinum obtained from a sputum sample of a South African HIV-infected patient with suspected pulmonary tuberculosis. Genomic DNA was extracted from three independent liquid cultures using the NucliSENS easyMAG (bioMérieux, France); in brief, a 200-l aliquot of the liquid culture was used as the input volume for DNA extraction using the generic protocol, with a final elution volume of 25 l.
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