Abstract
ABSTRACTA highly virulent strain of Francisella noatunensis subsp. orientalis, STIR-GUS-F2f7, was isolated from moribund red Nile tilapia (Oreochromis niloticus) farmed in Europe. In this communication, the complete genome sequencing of this bacterium is reported.
Highlights
A highly virulent strain of Francisella noatunensis subsp. orientalis, STIRGUS-F2f7, was isolated from moribund red Nile tilapia (Oreochromis niloticus) farmed in Europe
The genomic DNA of STIR-GUS-F2f7 was extracted as outlined by Seward et al (3)
The purity and concentration of the DNA were assessed by electrophoresis using a 1% agarose gel stained with ethidium bromide at 0.001%, and 260/280-to-260/230 ratios were determined using a NanoDrop ND1000 (Thermo Scientific, DE)
Summary
A highly virulent strain of Francisella noatunensis subsp. orientalis, STIRGUS-F2f7, was isolated from moribund red Nile tilapia (Oreochromis niloticus) farmed in Europe. The genomic DNA of STIR-GUS-F2f7 was extracted as outlined by Seward et al (3). The final DNA concentration was adjusted to 2 g and assessed with a Qubit 2.0 fluorometer (Invitrogen Life Technologies, Inc., CA).
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