Abstract
The genome of the streptomycin-resistant Citrobacter gillenii strain MBT-C3, isolated from lamb's lettuce in Germany, was sequenced. Sequence analysis showed the assembled draft genome size to be 5,167,205 bp, containing a predicted total of 5,011 protein-encoding genes, 8 rRNAs, and 71 tRNAs.
Highlights
The genome of the streptomycin-resistant Citrobacter gillenii strain MBTC3, isolated from lamb’s lettuce in Germany, was sequenced
The results showed that the C. gillenii MBT-C3 genome was distinct from the genomes of representative strains of related species
The whole-genome shotgun project of C. gillenii MBT-C3 has been deposited at DDBJ/ENA/GenBank under the accession no
Summary
The genome of the streptomycin-resistant Citrobacter gillenii strain MBTC3, isolated from lamb’s lettuce in Germany, was sequenced. The Citrobacter gillenii MBT-C3 strain was grown overnight in brain heart infusion (BHI) broth, and the genomic DNA was extracted using the ZR fungal/bacterial DNA miniprep kit (Zymo Research, Freiburg, Germany) and quantified using a Qubit 3 fluorometer (Invitrogen, Darmstadt, Germany). The Nextera XT DNA library preparation kit and the MiSeq reagent kit version 2 were used, according to the manufacturers’ instructions, using a MiSeq sequencer (Illumina, Munich, Germany).
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