Abstract
The genus Cellulophaga is composed of obligate aerobic Gram-negative bacteria commonly found in association with marine algae. We report the approximately 4.42-Mbp draft genome sequence of Cellulophaga sp. E6, which inhibits N-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-C12-HSL)–mediated quorum sensing (QS), lasB transcription, and biofilm formation by Pseudomonas aeruginosa.
Highlights
Recent reports have focused on potential applications for novel enzymes from Cellulophaga species that break down carrageenan [3, 10, 11]
Sequencing was carried out using a MiSeq Genome Sequencer (Illumina) at Tufts University Genomics Core, which generated 4,425,292 2 ϫ 250 bp paired-end reads. de novo assembly was done using CLC Genomics Workbench v7.0.4 (CLC Bio, Denmark) with the minimum contig size set to 250 bp, resulting in 72 contigs
The total size for the combined contigs is 4,420,065 bp, leading us to estimate the genome as approximately 4.42 Mb; it is predicted to contain 3,630 protein-coding genes, 36 tRNA genes, and 2 rRNA genes. 16S and 23S rRNA genes were detected, but since the assembly is based on short reads, the numbers and locations of multiple copies could not be determined
Summary
E6 [1, 2] from the surface of a coastal seaweed as part of a bioprospecting search for marine bacteria which have the potential to control P. aeruginosa virulence through inhibiting acylhomoserine lactone (AHL)based quorum sensing (QS). Cellulophaga spp. are obligate aerobes which hydrolyze agar and carrageenan. Recent reports have focused on potential applications for novel enzymes from Cellulophaga species that break down carrageenan [3, 10, 11]. Using a luciferase-based reporter construct (PlasB-luxCDABE) specific for 3-oxo-C12-HSL [12], we have shown that Cellulophaga sp.
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