Abstract

The Tara Oceans Consortium has published various metagenomes of marine environmental samples. Here, we report a contig of 2.6 Mbp from the assembly of a sample collected near the Marquesas Islands in the Pacific Ocean, covering a nearly complete novel verrucomicrobial genome. We propose the name "Candidatus Moanabacter tarae" for the corresponding bacterium.

Highlights

  • The Tara Oceans Consortium has published various metagenomes of marine environmental samples

  • Based on the presence of 131 out of 139 bacterial single-copy marker genes (9) and the genome completeness estimation method miComplete v0.2.0, which takes the general distances between these marker genes into account, the contig was estimated to cover 92% of the complete genome

  • A BLAST search of the 16S rRNA gene recovered several uncultured marine sediment bacteria (GenBank accession numbers FN553635, JF809735, AB694308, and AB694307; 91 to 92% identity), epixenosomes of Euplotidium arenarium (87% identity), and Coraliomargarita akajimensis (86% identity) as the top hits, demonstrating the verrucomicrobial origin and novel nature of the contig

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Summary

Introduction

The Tara Oceans Consortium has published various metagenomes of marine environmental samples. The longest contig in this assembly was 2,633,965 bp and had a GC content of 46%. Based on the presence of 131 out of 139 bacterial single-copy marker genes (9) and the genome completeness estimation method miComplete v0.2.0 (https://bitbucket.org/ evolegiolab/micomplete), which takes the general distances between these marker genes into account, the contig was estimated to cover 92% of the complete genome.

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