Abstract
Bacillus thuringiensis NBIN-866, a Gram-positive bacterium, was isolated from soil in China. We announce here the draft genome sequence of strain B. thuringiensis NBIN-866, which possesses highly nematocidal factors, such as proteins and small molecular peptides.
Highlights
Bacillus thuringiensis NBIN-866, a Gram-positive bacterium, was isolated from soil in China
The genome sequencing of B. thuringiensis NBIN-866 was performed with a strategy involving Solexa paired-end sequencing technology
Sequencing was performed with the paired-end strategy of 502-bp reads to produce 645 Mb and 294 Mb of filtered sequences, respectively, representing 170-fold coverage, with an Illumina Solexa Genome Analyzer (GA) IIx (Wuhan Yanxing Biotechnology Co., Ltd., Wuhan, China), and the reads were assembled into 203 contigs and 162 scaffolds using the Short Oligonucleotides Alignment Program (SOAP) de novo alignment tool
Summary
Bacillus thuringiensis NBIN-866, a Gram-positive bacterium, was isolated from soil in China. Bacillus thuringiensis is a Gram-positive spore-forming soil bacterium with the ability to produce insecticidal crystal proteins [1,2,3,4]. It shows toxicity to certain insect species [5, 6] and has been widely used as a biopesticide for controlling agricultural pests. B. thuringiensis can produce multiinsecticidal metabolites, like insecticide crystal proteins [7], cytotoxin [8], vegetative insecticidal protein [9], secret insecticidal protein [10], thuringiensin [11], zwittermicin A [12], Mtx-like toxin [13], Bin-like toxin [14], and more.
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