Abstract
Methanotrophic bacteria inhabit a wide range of natural (e.g. wetlands, lakes and soils) and anthropogenic (e.g. wastewater treatment plants and landfills) environments. They play a crucial role in mitigating atmospheric emissions of the greenhouse gas methane. There is also a growing interest in applying methanotrophs in the bioconversion of biogas - and natural gas - methane into value-added products (e.g. chemicals and single-cell protein). Hence, isolation and genome sequencing of methanotrophic bacteria is needed to provide important data on their functional capabilities. Here, we describe the de novo assembled draft genome sequences of Methylovulum psychrotolerans strain S1L isolated from hypoxic water column layer of boreal Lake Lovojärvi (Southern Finland), comprising total of 5090628 bp in 11 contigs with G+C – content of 50.9% and containing 4554 coding sequences. The draft genome of strain S1L represents the first published genome of M. psychrotolerans strain isolated from lake ecosystems. In addition, we present the genome sequence of Methylomonas paludis strain S2AM, isolated from water column of boreal Lake Alinen Mustajärvi (Southern Finland), comprising 3673651 bp in 1 contig with G+C – content of 48.2% and 3294 coding sequences. The draft genome of strain S2AM represents the first published genome of M. paludis. The preliminary genome annotation analysis of both S1L and S2AM identified genes encoding oxidation of methane, methanol, formaldehyde and formate, assimilation of carbon, ammonium and nitrate, N2 fixation, as well as various enzymes enabling the survival in hypoxic conditions, i.e. high-affinity oxidase, hemerythrins, fermentation enzymes (for production of acetate, succinate and H2) and respiratory nitrite reductases. The draft genomes have been deposited at GenBank under the accession JAGVVN000000000 for S1L and CP073754 for S2AM.
Highlights
Draft genome sequence data of methanotrophic Methylovulum psychrotolerans strain S1L and Methylomonas paludis strain S2AM isolated from hypoxic water column layers of boreal lakes
We describe the de novo assembled draft genome sequences of Methylovulum psychrotolerans strain S1L isolated from hypoxic water column layer of boreal Lake Lovojärvi (Southern Finland), comprising total of 5090628 bp in 11 contigs with G+C – content of 50.9% and containing 4554 coding sequences
The draft genome of strain S1L represents the first published genome of M. psychrotolerans strain isolated from lake ecosystems
Summary
Biological sciences Bacterial Genomics, Applied Microbiology and Biotechnology, Biogeochemistry, Environmental Microbiology Genomic sequence Table Figure Whole genome sequencing using Illumina NovaSeq 6000 platform for short reads and Sequel SMRT Cell 1M v2 for long reads. Raw Assembled/analyzed Strains S1L and S2AM were isolated from hypoxic boreal lake water samples. DNA extraction, sequencing library preparation, short and long read sequencing, raw data filtering, draft de novo assembly and annotation procedures. Institution: Tampere University City/Town/Region: Tampere Country: Finland Isolation sources: Strain S1L: Lake Lovojärvi (61° 04 N, 25° 02 E) Strain S2AM: Lake Alinen Mustajärvi (61° 12 N, 25° 06 E) Repository: The assembled whole-genome shotgun data (.fasta) has been deposited at GenBank under the accession numbers JAGVVN000000000 for S1L (https://www.ncbi.nlm.nih.gov/nuccore/JAGVVN000000000) and CP073754 for S2AM (https://www.ncbi.nlm.nih.gov/nuccore/CP073754). This data can be used in predicting the function of methanotrophs in natural ecosystems under variating physicochemical conditions as well as in developing methane-based bioproduct platforms
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