Abstract
The poultry red mite, Dermanyssus gallinae, is a major worldwide concern in the egg-laying industry. Here, we report the first draft genome assembly and gene prediction of Dermanyssus gallinae, based on combined PacBio and MinION long-read de novo sequencing. The ∼959-Mb genome is predicted to encode 14,608 protein-coding genes.
Highlights
The poultry red mite, Dermanyssus gallinae, is a major worldwide concern in the egg-laying industry
Full-length transcripts were obtained from the total RNA from mixed-stage D. gallinae and were analyzed with PacBio Iso-Seq pipelines within the PacBio single-molecule real-time (SMRT) Portal v5.0.1.10424 [7], generating 13,612 high-quality and 53,082 low-quality isoforms post-Quiver polishing
BLAST hits against the NCBI nonredundant database (July 2018) were identified for 13,840 genes, and Gene Ontology (GO), performed in Blast2GO [10], resulted in the assignment of GO terms for 11,624 genes and functional annotation of 10,914 genes
Summary
The poultry red mite, Dermanyssus gallinae, is a major worldwide concern in the egg-laying industry. Nanopore Technologies MinION reads (6 gigabases [Gb] of sequence data generated with the 1D ligation kit on an R9.4 flow cell) using PBJelly 2 [6] followed by 8 iterations of genome polishing with Arrow [7]. The final assembly contained 7,171 contigs with an N50 value of 278,630 bp and an L50 value of 800 contigs, the largest scaffold having 3,781,415 bp and an overall genome GC content of 44.6%.
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