Abstract
ObjectiveLncRNA SOX2-OT is involved in a variety of cancers. This study explored the effect of lncRNA SOX2-OT on hepatocellular carcinoma (HCC) cells.MethodsSOX2-OT expressions were detected in HCC tissues and normal tissues, normal cells, and HCC cells. The relationship between SOX2-OT and prognosis was analyzed by TCGA. After SOX2-OT expression was inhibited using siRNA, HCC cell malignant behaviors were evaluated. The subcellular localization of SOX2-OT in HCC cells was predicted and analyzed. The binding relationships among SOX2-OT, miR-143-3p, and MSI2 were analyzed by bioinformatics website, dual-luciferase assay, and RNA pull-down assay. The effect of miR-143-3p and MSI2 on the regulation of SOX2-OT on biological behaviors of HCC cells was confirmed by functional rescue experiments. The effect of SOX2-OT on the tumorigenicity of HCC was evaluated by subcutaneous tumorigenesis in nude mice.ResultsSOX2-OT was highly expressed in HCC cells and tissues. The prognosis was poor in HCC patients with high SOX2-OT expression. Downregulating SOX2-OT inhibited HCC cell malignant behaviors. SOX2-OT bound to miR-143-3p to promote MSI2 expression. Downregulating miR-143-3p or upregulating MSI2 averted the role of si-SOX2-OT in HCC cells. Nude mouse subcutaneous tumorigenesis showed that SOX2-OT downregulation decreased the tumorigenicity of HCC, and affected the levels of miR-143-3p and MSI2 mRNA in tumor tissues.ConclusionSOX2-OT inhibited the targeted inhibition of miR-143-3p on MSI2 through competitively binding to miR-143-3p, thus promoting MSI2 expression and proliferation, invasion, and migration of HCC cells.
Highlights
Primary carcinoma of the liver is one of the most prevalent cancers and a most common cause of cancer mortality all over the world [1]
This study explored the effect of lncRNA SOX2-OT on hepatocellular carcinoma (HCC) cells
SOX2-OT inhibited the targeted inhibition of miR-143-3p on MSI2 through competitively binding to miR-143-3p, promoting MSI2 expression and proliferation, invasion, and migration of Hepatocellular carcinoma (HCC) cells
Summary
Primary carcinoma of the liver is one of the most prevalent cancers and a most common cause of cancer mortality all over the world [1]. The incidence rate of liver cancer and liver cancer-related deaths has been increasing for decades [2]. Hepatocellular carcinoma (HCC) accounts for the majority of primary liver malignancies [3]. The development of HCC is related to chronic viral infection (mainly HBV and HCV). HCC is characterized by multicentric cancerization, intrahepatic metastasis, and multistage cancerization [4]. As HCC arises in the body of chronic liver disease, its prognosis is determined by the underlying disease, and the therapeutic options are limited in late stages [5]. The need to clarify the precise mechanisms of HCC development is very strong [6]
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