Abstract
BackgroundLaryngeal squamous cell carcinoma (LSCC) is considered to be a common malignancy of the head and neck with poor prognosis for its late diagnosis, metastasis and recurrence. Growing evidence demonstrates that the dysregulation of miR-29c-3p (microRNA-29c-3p) plays an important role in various tumor processes. Our study investigates the expression of miR-29c-3p in LSCC and analyzes the correlation of its dysregulation with clinicopathologic parameters and prognosis.MethodsThe expression of hsa-miR-29c-3p in LSCC tissues and the adjacent normal laryngeal tissues was detected in 96 LSCC formalin-fixed paraffin-embedded tissues by quantitative real-time PCR (qRT-PCR). The SPSS statistical software package (17.0) was used to analyze the associations between miR-29c-3p expressions and various clinicopathological characteristics. The overall survival (OS) was analyzed by the Kaplan-Meier method and log-rank test, and we analyzed the independent factor of prognosis by Cox proportional hazard analysis.ResultsA downregulation of miR-29c-3p expression in LSCC was significantly correlated with smoking index, tumor size, tumor site, differentiation, T classification, TNM stage, and lymph node metastasis (P < 0.05), but there was no correlation with age and alcohol consumption (P > 0.05). In the multivariate survival analysis, low miR-29c-3p expression was associated with shorter overall survival (P < 0.05). Furthermore, miR-29c expression was an independent prognostic factor for laryngeal cancer patients.ConclusionsMiR-29c-3p has different expression levels at different stages of tumor progression, suggesting that miR-29c-3p may be a promising biomarker for evaluating the progression of LSCC and the prognosis of patients with LSCC. MiR-29c-3p can also be a novel molecular target for anti-laryngeal cancer therapy.
Highlights
Laryngeal squamous cell carcinoma (LSCC), as the most common histopathological type of laryngeal cancer, is the second-most common malignancy of the head and neck [1, 2].According to studies, almost 3650 people died from LSCC worldwide in 2012, concomitantly with the increasing incidence and mortality of LSCC year-by-year [3]
Downregulation of miR-29c in LSCC The results of quantitative real-time PCR (qRT-PCR) showed that the relative expression level of miR-29c in the 66 patients with LSCC was miR-29c-3p expression (X Æ S)
Our results indicated that low level expression of miR-29c correlates with smoking index≥400, which implied that miR-29c might be involved in the pathomechanism of cigarettes inducing laryngeal cancer
Summary
Laryngeal squamous cell carcinoma (LSCC), as the most common histopathological type of laryngeal cancer, is the second-most common malignancy of the head and neck [1, 2].According to studies, almost 3650 people died from LSCC worldwide in 2012, concomitantly with the increasing incidence and mortality of LSCC year-by-year [3]. MiRNAs play important roles in all tumor-related processes, including proliferation, differentiation, apoptosis, metastasis, invasion and angiogenesis in LSCC. MiR-29c-3p, as a tumor suppressor in the miRNAs family [9], has been demonstrated to be downregulated in many solid tumors, such as nasopharyngeal carcinoma [10], gastric cancer [11], hepatocellular carcinoma [12], gallbladder cancer [13], esophageal cancer [14], breast cancer [15], colon cancer [16] and head and neck cancers [17], which revealed that lower expression of miR-29c was associated with lymph node metastasis, tumor differentiation, TNM stage and poor prognosis. Laryngeal squamous cell carcinoma (LSCC) is considered to be a common malignancy of the head and neck with poor prognosis for its late diagnosis, metastasis and recurrence. Our study investigates the expression of miR-29c-3p in LSCC and analyzes the correlation of its dysregulation with clinicopathologic parameters and prognosis
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