Abstract
The present study aimed to unveil the biological role of carbonic anhydrase IV (CA IV) and its association with clinical pathological characteristics and prognostic significance in non-small cell lung cancer (NSCLC). The relative expression level of CA IV was measured by reverse transcription-quantitative polymerase chain reaction in 114 resected NSCLC tumors and matched adjacent normal tissues and NSCLC cell lines. Overexpression and cell proliferation were assessed in human NSCLC cell lines transfected with CA IV mRNA by lentivirus-mediated technology. The association of CA IV expression with clinical pathological features and overall survival in 114 cases of NSCLC patients was analyzed. It was demonstrated that CA IV expression was significantly downregulated in NSCLC tumors and six cell lines. Reduced expression of CA IV was significantly correlated with lymph node metastasis. The overall survival of NSCLC patients with low CA IV expression was significantly shorter compared with the high expression group. Overexpression of CA IV suppressed cell proliferation in A549 and NCI-H1299 cells. The results indicate that low expression of CA IV promotes cell proliferation and serves as an indicator for poor prognosis in NSCLC.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
