Down-Regulation of circulating miR-23a-3p, miR-101-3p, and miR-let-7c in Women with Idiopathic Recurrent Pregnancy Loss.

Abstract

Recently, circulating microRNAs have attracted much attention because they can serve as reliable, non-invasive diagnostic biomarkers for human diseases. This study aimed to quantify miR-23a-3p, miR-101-3p, and miR-let-7c expression levels in plasma samples of patients with idiopathic recurrent pregnancy loss (iRPL) and healthy subjects and to evaluate their potential diagnostic value in diagnosis of iRPL. A total of 120 plasma samples were obtained from sixty women with a history of iRPL and sixty healthy fertile women to evaluate the expression levels of the circulating miR-23a-3p, miR-101-3p, and miR-let-7c by quantitative real-time polymerase chain reaction (qPCR) technique. The correlation between miR-23a-3p, miR-101-3p, and miR-let-7c and clinicopathological parameters was also assessed. Receiver operating characteristic (ROC) curve was plotted to determine the diagnostic accuracy of studied miRNAs in iRPL. Our results showed that the miR-23a-3p expression level in plasma of iRPL patients was lower than those in healthy controls but without a statistically significant difference (p = 0.113). The expression levels of miR-101-3p and miR-let-7c were significantly downregulated in iRPL patients compared with healthy subjects (p < 0.05). The plasma levels of miR-23a-3p and miR-let-7c were negatively correlated with number of abortions in iRPL patients. We observed statistically significant positive correlation between miR-23a-3p and miR-101-3p (r = 0.478, p = 0.001), miR-23a-3p and miR-let-7c (r = 0.561, p = 0.0001), and miR-101-3p and miR-let-7c (r = 0.533, p = 0.0001) in patients with iRPL. The current study provides evidence indicating that downregulation of miR-23a-3p, miR-101-3p, and miR-let-7c may be associated with iRPL.