Abstract
Southern rice black-streaked dwarf virus (SRBSDV), a new virus from Fiji, has seriously damaged rice crops in southern China and northern Vietnam in recent years. This virus is difficult to diagnose in the early stages of infection, and is very destructive at the late stage. In the present study, a dot immunobinding assay (DIBA) that has a high sensitivity for diagnosing SRBSDV was developed. Two kinds of treatment for the DIBA were evaluated to determine the most effective one for removing chlorophyll interferences via rice extraction. The first included several reagents to remove chlorophyll, namely, the alkaline reagents like magnesium oxide and alumina oxide, the adsorbent reagents like activated carbon and bentonite, as well as the extraction agent acetone. The second and third treatments, which were used to remove chlorophyll in blot membrane-nitrocellulose and polyvinylidene fluoride (PVDF), included several organic solvents containing methanol, ethanol, acetone, ethyl acetate, and diethyl ether. The results showed that activated carbon and methanol yielded the best contrasting purple color for the infected samples by decreasing the chlorophyll content.
Highlights
Southern rice black-streaked dwarf virus (SRBSDV), which causes a malignant disease in rice, has become prevalent in recent years
Chlorophyll in the precipitates was clearly observable on the magnesium oxide (MgO), Al2O3, and activated carbon groups
A dot immunobinding assay (DIBA) for removing chlorophyll that has a high sensitivity for diagnosing the SRBSDV was developed
Summary
Southern rice black-streaked dwarf virus (SRBSDV), which causes a malignant disease in rice, has become prevalent in recent years. Some studies have reported successful SRBSDV detection methods using reverse transcription-polymerase chain reaction (RT-PCR) and loop-mediated isothermal amplification (LAMP)-PCR against the gene of SRBSDV [2,4,5,6,7,8,9]. These laboratory methods are scientific and accurate, but rather laborious, time consuming, complicated, and expensive. These methods are not readily available in rice cultivating areas, especially in rural villages
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