Abstract
Cystic hydatid disease (Hydatidosis) is one of the most important parasitic zoonoses and remains a public health and economic problem all over the world. Cyst fluid was obtained from hepatic and pulmonary cysts for demonstration of protoscolices and hooklets. Therefore, a standardized and approachable diagnostic tool for the serodiagnosis of CE is still needed. Dot-ELISA is a solid phase diagnostic method for detection of antigen or antibody that is used widely for diagnosis of protozoan and metazoan diseases of human and animals. In the present study, E. granulosus protoscolex antigen was early detected in patient sera using Dot-ELISA, PAb was prepared from anti-rabbit sera and used for coating and as conjugate in Dot-ELISA technique. 48 patients out of 50 were positive to E. spp. with sensitivity and specificity 96% and 94%, respectively. The PPV was 94% and NPV was 90%. Finally, the present results showed that the Dot-ELISA was easy to perform, not expensive, safe, and with good sensitivity and specificity.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
