Dose-Dependent Increase in Whole Blood Omega-3 Fatty Acid Concentration in Horses Receiving a Marine-Based Fatty-Acid Supplement

Abstract

The objective of this study was to determine the effects of an oral, commercially available, marine based omega-3 fatty acid (n3-FA) supplement on fatty acid characteristics in horse whole blood. Fifty healthy, adult horses of various light breeds were assigned to one of two treatment groups: Group 1 receiving 7.5g/day of the test supplement, and Group 2 receiving 15g/day of the test supplement. The supplement contained 0.092g docosahexaenoic acid (DHA) and 0.148g eicosapentaenoic acid (EPA) per gram. Therefore, Group 1 received 1.11g of EPA and 0.69g of DHA daily, while Group 2 received 2.22g of EPA and 1.38g DHA daily. Blood was taken at time of enrollment and after 6 and 12 weeks of supplementation. Blood was subjected to gas chromatography to quantify the fatty acid characteristics of whole blood. At both 6 and 12 weeks following supplementation, there was a significant increase in all n3-FAs evaluated, including DHA and EPA, compared to baseline values, with Group 2 significantly increased compared to Group 1 at both time points. There was also a significant decrease in omega-6 fatty acids (n6-FAs) between baseline and 6 weeks of supplementation in both groups, with a larger decrease seen in Group 2. The dose-dependent increases in concentration of all n3-FAs evaluated at all-time points validates the use of this product as a n3-FA targeted supplement in horses. These findings also suggest that dose of supplement has a greater effect on increasing whole blood n3-FAs compared to duration of treatment.