Abstract
Objective To investigate the machenism of donor liver natural killer(NK) cells alleviating actue rejection after liver transplantation.Methods Lewis rats wereused as liver graft donors and BN rats as recipients,Lewis-BN as acute rejection model was established by the modified two-cuff method.The donor leukocytes were depleted by total body irradiation of 60Co source,and the donor passenger leukocyte immunity were reconstituted by transfusion donor liver NK cells via portal vein during the course of transplantation of the irradiate liver.Three various transplantation groups were designed:Group A.acute rejection group (Lewis-BN),n =18,Group B.60Co irradiation acute rejection group (60Co-Lewis-BN),n =18,C.60Co irradiation + donor liver NK cells transfusionvia portal vein acute rejection group (60CoLewis-BN + donor NK cells).The recipients were sacrificed day7 posttransplantation respectively,TGF-β1level in peripheral blood,arginase-1 (Arg-1) protein expression in liver tissues and histo pathological of liver grafts were observed and detected.Results Acute rejection in B group was more severe than A group,peripheral blood TGF-β1 levels [(28.00 ± 6.08) μg/L vs.(11.00 ± 0.57) μg/L,P ＜ 0.01] and Arg-1 expression (0.687 ± 0.040 vs.0.487 ± 0.030,P ＜ 0.05) of the liver in group B were significantly lower than group A; But compared with B group,the C group alleviate the actue rejection donor following liver NK cells transfution via portal vein,and the peripheral blood TGF-β1 levels [(11.00 ±0.57) μg/L vs.(27.60 ± 1.45) μ.g/L,P ＜ 0.05] and Arg-1 expression (0.487 ± 0.030 vs.0.605 ± 0.080,P ＜ 0.05)of the liver in group C group were significantly lower than group B.Conclusion Donor liver NK cells can alleviate liver allograft acute rejection,the machenism may be associated with up-regulation expression of Arg-1 in the liver. Key words: Donor liver natural killer cells; Arginase-1 ; Acute rejection; Liver transplantation; Rat
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