Abstract
Background In most vertebrates organ asymmetries arise in early development through the left-right organizer (LRO). LRO ciliated cells induce a leftward fluid flow responsible for the asymmetric expression of Nodal pathway genes in tissues that will originate internal organs. We have recently published that charon/dand5 transcription is an early flow target in zebrafish by being expressed in the LRO, first symmetrically and later asymmetrically to the right where the fluid flow is weaker. We showed that absence of flow originates symmetric charon expression [1]. Pkd2 has been reported as part of a mechanosensor complex that senses flow and induces a calcium inward flux in kidney cells [2] and mouse LRO [3]. In agreement, mouse and zebrafish Pkd2 mutants have LR defects [4,5].
Highlights
In most vertebrates organ asymmetries arise in early development through the left-right organizer (LRO)
We will show if charon/dand[5] quantification by qRT-PCR is similar
When both flow and Pkd[2] are impaired, 80% of embryos showed symmetric charon/dand[5], which indicates a synergistic effect
Summary
In most vertebrates organ asymmetries arise in early development through the left-right organizer (LRO). LRO ciliated cells induce a leftward fluid flow responsible for the asymmetric expression of Nodal pathway genes in tissues that will originate internal organs. We have recently published that charon/dand[5] transcription is an early flow target in zebrafish by being expressed in the LRO, first symmetrically and later asymmetrically to the right where the fluid flow is weaker. We showed that absence of flow originates symmetric charon expression [1]. We will show if charon/dand[5] quantification by qRT-PCR is similar. When both flow and Pkd[2] are impaired, 80% of embryos showed symmetric charon/dand[5], which indicates a synergistic effect
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