Do Ischemic Preconditioning and Propofol Have Synergistic Effect on Reducing Adhesion of Polymorphonuclear Neutrophils?

Abstract

Background: Ischemic preconditioning (IPC) and propofol has been demonstrated to inhibit postischemic adhesion of PMNs on coronary vascular endothelium, respectively. The purpose of this study is to investigate whether IPC and propofol inhibit postischemic coronary vascular adhesion of PMNs synergistically. Methods: Twenty-eight isolated rat hearts were subjected to stabilization for 15 min, perfusion for 15 min, global ischemia for 15 min and reperfusion for 60 min serially. The isolated hearts were divided into 4 groups as follows: control group, PMNs were infused for 1 min on 1 min after reperfusion following global ischemia; IPC group, IPC was performed before global ischemia and PMNs were infused as the same manner; PPF group, Propofol (0.3g/ml) was infused from perfusion until 5 min after reperfusion and PMNs were infused as the same manner; IPC/PPF group, IPC was performed before global ischemia and propofol was infused from perfusion until 5 min after reperfusion and PMNs were infused as the same manner. PMNs adhesion, heart rate, left ventricular developed pressure, dP/dt, coronary flow and creatinine kinase enzyme were measured. Results: Postischemic PMNs adhesion was reduced in IPC, propofol, and IPC/PPF group compared to the control group (30.8 7.3%, 32.0 3.9%, 20.9 6.1% vs 75.7 6.3%, P