Do entomopathogenic nematodes induce immune priming?

Abstract

Although the study of immune priming in insects is a growing area of research, its occurrence in various biological models has not been evaluated, and its mechanisms are poorly understood. Whether entomopathogenic nematodes (EPNs) can induce immune priming and what role their virulence might play in it has not been assessed. Here, we tested for the first time: 1) whether a nematode is capable of eliciting immune priming, and 2) whether nematode virulence affects immune priming. Host larvae of Tenebrio molitor were first exposed to one of two EPN strains (low or high virulence). They were then exposed again to a challenge (high) dose of their respective strain, and their survival was recorded. Based on current literature, we expected that host larvae primed with a low-virulence strain would not show immune priming but that those exposed to a high-virulence strain would. Instead, we found that host larvae primed with either strain did not exhibit immune priming. Further, the survival of the hosts primed with the highly virulent strain was significantly reduced relative to the control group, and no measurable immune priming was found, as also indicated by resting metabolic rate (production of CO2). Future research is needed to determine whether virulence-associated bacteria underlie this lowered survival and/or whether another factor, such as immune evasion strategies, is related to these results.